Human Motility Related Protein (MRP1) ELISA Kit

SKU: orb777160

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Motility Related Protein(MRP1).

Research Area

Musculoskeletal & Connective Tissue Research, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MRP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MRP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MRP1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	13.4 pg/mL
Concentration	2000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD9 ELISA Kit, TSPAN29 ELISA Kit, 5H9 ELISA Kit, BA2 ELISA Kit, BTCC-1 ELISA Kit, DRAP-27 ELISA Kit, GIG2 ELISA Kit, MIC3 ELISA Kit, P24 ELISA Kit, Tetraspanin 29 ELISA Kit, Cell growth-inhibiting gene 2 protein ELISA Kit, Leukocyte antigen MIC3 ELISA Kit

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Human Motility Related Protein (MRP1) ELISA Kit

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UniProt

P21926

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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