Human Mucin 1 (MUC1) ELISA Kit

SKU: orb775066

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 1(MUC1).

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Tested Applications

ELISA

Application Notes

standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MUC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MUC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MUC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MUC1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.63-40 ng/mL
Sensitivity	0.276 ng/mL
Concentration	40 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD227 ELISA Kit, EMA ELISA Kit, H23AG ELISA Kit, KL-6 ELISA Kit, PEM ELISA Kit, PEMT ELISA Kit, PUM ELISA Kit, CA15-3 ELISA Kit, Cancer antigen 15-3 ELISA Kit, Carcinoma-associated mucin ELISA Kit, Episialin ELISA Kit, Peanut-reactive urinary mucin ELISA Kit, Polymorphic epithelial mucin ELISA Kit

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UniProt

P15941

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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