Human N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit

SKU: orb775781

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acetyl Beta-D-Glucosaminidase(NAGase).

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Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NAGase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NAGase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NAGase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NAGase in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Urine
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.54 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MGEA5 ELISA Kit, MEA5 ELISA Kit, NCOAT ELISA Kit, Hexosaminidase C ELISA Kit, N-Acetylhexosaminidase ELISA Kit, Hyaluronidase ELISA Kit, Meningioma-Expressed Antigen 5 ELISA Kit, Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase ELISA Kit

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Human N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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