Human Neuregulin 1 (NRG1) ELISA Kit

SKU: orb776096

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuregulin 1(NRG1).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NRG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NRG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NRG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NRG1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.054 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ARIA ELISA Kit, GGF ELISA Kit, HGL ELISA Kit, HRG ELISA Kit, HRGA ELISA Kit, NDF ELISA Kit, SMDF ELISA Kit, Acetylcholine receptor-inducing activity ELISA Kit, Glial growth factor ELISA Kit, Heregulin ELISA Kit, Neu differentiation factor ELISA Kit, Sensory and motor neuron-derived factor ELISA Kit

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UniProt

Q02297

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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