Human Nuclear Antigen Antibody

SKU: orb248661

Description

This human nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Images & Validation

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Tested Applications	FACS, ICC, IF, IHC-Fr, IP
Dilution range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunocytochemistry (acetone-fixed cells): 1-2ug/ml for 30 min at RT,Immunohistochemistry (Frozen): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nuclear Antigen antibody to be titered up or down for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	235-1
Immunogen	Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear Antigen antibody.

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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ICC staining of paraformaldehyde-fixed human HeLa cells with Nuclear Antigen antibody (red, clone 235-1) and counterstained with DyLight 488 conjugated Phalloidin (green).

IHC testing of acetone-fixed frozen human tonsil tissue with Nuclear Antigen antibody (clone 235-1).

FACS testing of MCF-7 cells: Black = no Ab; Green = isotype control; Red = Nuclear Antigen antibody PE conjugate (V2345PE)

FACS testing of HeLa cells: Black = no Ab; Green = isotype control; Red = Nuclear Antigen antibody PE conjugate (V2345PE)

Flow cytometry testing of PFA-fixed human MCF7 cells with Human Nuclear Antigen antibody (clone 235-1); Red = isotype control, Blue = Human Nuclear Antigen antibody.

Flow cytometry testing of PFA-fixed human HeLa cells with Human Nuclear Antigen antibody (clone 235-1); Red = isotype control, Blue = Human Nuclear Antigen antibody.

Flow cytometry testing of PFA-fixed human Jurkat cells with Human Nuclear Antigen antibody (clone 235-1); Red = isotype control, Blue = Human Nuclear Antigen antibody.

Flow cytometry testing of PFA-fixed human K562 cells with Human Nuclear Antigen antibody (clone 235-1); Red = isotype control, Blue = Human Nuclear Antigen antibody.

ICC staining of paraformaldehyde-fixed human K562 cells with Nuclear Antigen antibody (green, clone 235-1) and counterstained with Phalloidin (red).

ICC staining of paraformaldehyde-fixed human MCF7 cells with Nuclear Antigen antibody (green, clone 235-1) and counterstained with Phalloidin (red).

Flow cytometry testing of PFA-fixed human Raji cells with Human Nuclear Antigen antibody (clone 235-1); Red = isotype control, Blue = Human Nuclear Antigen antibody.

SDS-PAGE analysis of purified, BSA-free Nuclear Antigen antibody (clone 235-1) as confirmation of integrity and purity.

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Protocol Information

IHC-Fr

Immunohistochemistry Frozen

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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ICC

Immunocytochemistry

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Immunoprecipitation

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