Human Osteoprotegerin (OPG) ELISA Kit

SKU: orb775041

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteoprotegerin(OPG).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OPG in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.058 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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TNFRSF11B ELISA Kit, TNFRSF11-B ELISA Kit, OCIF ELISA Kit, OPG ELISA Kit, TR1 ELISA Kit, Tumor Necrosis Factor Receptor Superfamily Member 11b ELISA Kit, Osteoclastogenesis Inhibitory Factor ELISA Kit

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UniProt

O00300

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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