Human PCSK9 Protein, His Tag

SKU: orb257737

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Description

Human PCSK9, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).

Images & Validation

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Application Notes

This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.2 kDa respectively. The protein migrates as 17 kDa and 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	PCSK9
Reactivity	Human
Tag	C-10×His
Molecular Weight	13.8 kDa and 59.2 kDa
Expression Region	Gln 31 - Gln 692
Purity	90%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 24 months in lyophilized state;. -70°C for 24 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

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SDS-PAGE analysis of Human PCSK9 protein

Line graph illustrates about the sensitivity range of Human PCSK9 protein using different concentrations

Quick Database Links

Gene Symbol

PCSK9

UniProt

Q8NBP7

UniProt Details

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No UniProt data available

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