Human Peptidyl Arginine Deiminase Type IV (PADI4) ELISA Kit

SKU: orb777768

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type IV(PADI4).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PADI4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PADI4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PADI4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PADI4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.119 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PAD ELISA Kit, PADI5 ELISA Kit, PDI4 ELISA Kit, PDI5 ELISA Kit, Peptidyl Arginine Deiminase Type V ELISA Kit, Peptidylarginine deiminase IV ELISA Kit

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Human Peptidyl Arginine Deiminase Type IV (PADI4) ELISA Kit

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UniProt

Q9UM07

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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