Human Phospholipase A2, Calcium Independent (iPLA2) ELISA Kit

SKU: orb776223

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Calcium Independent(iPLA2).

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Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human iPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human iPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human iPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human iPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	30 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PLA2G6 ELISA Kit, CaI-PLA2 ELISA Kit, GVI ELISA Kit, INAD1 ELISA Kit, PNPLA9 ELISA Kit, Ca2+ Independent PLA2 ELISA Kit, 85 kDa Calcium-Independent Phospholipase A2 ELISA Kit, Phospholipase A2,group VI(Cytosolic,Calcium-Independent) ELISA Kit

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Human Phospholipase A2, Calcium Independent (iPLA2) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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