Human Plasmin/Antiplasmin Complex (PAP) ELISA Kit

SKU: orb2668020

Description

Research Area

Hematology, Protein Biochemistry, Signal Transduction

Images & Validation

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Application Notes

standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	plasma
Assay Type	Sandwich
Assay Time	3.5h
Range	7.81-500 ng/mL
Sensitivity	2.3 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Plasmin/Antiplasmin Complex

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