Human Platelet Derived Growth Factor D (PDGFD) ELISA Kit

SKU: orb776839

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor D(PDGFD).

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 800 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDGFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDGFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDGFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDGFD in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	12.5-800 pg/mL
Sensitivity	4.9 pg/mL
Concentration	800 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PDGF-D ELISA Kit, IEGF ELISA Kit, SCDGF-B ELISA Kit, Spinal Cord Derived Growth Factor B ELISA Kit, Iris-expressed growth factor ELISA Kit, Platelet-derived growth factor D, receptor-binding form/latent form ELISA Kit

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Human Platelet Derived Growth Factor D (PDGFD) ELISA Kit

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UniProt

Q9GZP0

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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