Human PLAU Protein, His Tag

SKU: orb613660

Description

Human PLAU, His Tag (activated by trypsin) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # P00749-1).

Images & Validation

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Application Notes

This protein carries a polyhistidine tag at the C-terminus. The active form of Human PLAU is a disulfide-linked heterodimer composed of long chain A (Ser 21 - Phe 177) and chain B (Ile 179 - Leu 431) with calculated MW of 17.8 kDa and 29.2 kDa. The long chain A is further cleaved to yield a short chain A (Lys 156 - Phe 177) and N-terminal fragment (Ser 21 - Lys 155) with calculated MW of 15.3 kDa. The protein migrates as 31-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	PLAU
Reactivity	Human
Tag	C-6×His
Molecular Weight	15.3 kDa, 17.8 kDa and 29.2 kDa
Expression Region	Ser 21 - Leu 431
Purity	90%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	25 mM HEPES, 150 mM NaCl, pH 7.5
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

PLAU

UniProt

P00749

UniProt Details

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No UniProt data available

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