Human Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit

SKU: orb775300

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy Associated Plasma Protein A(PAPPA).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAPPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAPPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAPPA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.12 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PAPP-A ELISA Kit, PAPA ELISA Kit, ASBABP2 ELISA Kit, DIPLA1 ELISA Kit, IGFBP-4ase ELISA Kit, PAPPA1 ELISA Kit, Pappalysin 1 ELISA Kit, Insulin-Like Growth Factor Binding Protein-4 Protease ELISA Kit, Differentially Placenta 1 Expressed Protein ELISA Kit

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Human Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit

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UniProt

Q13219

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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