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Description
Research Area
Disease Biomarkers
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Target | R-Spondin 1 |
| Reactivity | Human |
| Tag | C-Fc |
| Expression Region | Ser 21 - Ala 146 |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
| MW | 40.2 kDa |
| Purity | 95% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | 52 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Human RSPO1(21-146) Protein, hFc Tag [orb1290875]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 39.9 kDa after removal of the signal peptide. The apparent molecular mass of RSPO1(21-146)-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg Recombinant Human RSPO3 Protein [orb3002398]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Predicted: 47.9 KDa. Observed: 61 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman RSPO3 protein [orb392402]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Human cells
10 μg, 50 μg, 500 μg
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide