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Description
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:60,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Human Serum Albumin |
| Target | ALB |
| Purity | Anti-HUMAN SERUM ALBUMIN Antibody (Biotin Conjugated) was prepared from monospecific antiserum by immunoaffinity chromatography using purified human serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000); Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-human serum albumin antibody biotin conjugation, biotin conjugated rabbit anti-HSA antibody, biotin conjugated rabbit anti-human serum albumin antibody, rabbit anti-HSA antibody biotin conjugation, Analbuminemia antibody, rabbit anti-serum albumin antibody, rabbit anti-albumin antibody
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ELISA Results of Rabbit Anti-HSA Biotin Conjugated Antibody tested against purified human serum albumin. Each well was coated in duplicate with 1.0 µg of HSA. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugate stabilizer, Streptavidin-HRP conjugated (p/n orb348767) and TMB substrate (p/n orb348651).
Western Blot of Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated. Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000468.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ALB Antibody (Biotin) (orb2652781)
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