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Human Siglec-7 Protein, Fc Tag

SKU: orb1152432

Description

Human Siglec-7, Fc (L117A, L118A) Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).

Research Area

Immunology & Inflammation

Images & Validation

Application Notes
This protein carries a Fc (L117A, L118A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L117A, L118A/EU number: L234A/L235A) in human immunoglobulin G1 (hIgG1) Fc diminish binding to FcγRI leading to a significant reduction in ADCC and CDC in comparison to wild type human IgG1.

Key Properties

Expression SystemHEK293
TargetSiglec-7
ReactivityHuman
TagC-Fc(A2)
Expression RegionGln 19 - Leu 353
Endotoxins0.01 EU per μg
ConjugationUnconjugated
MW63.3 kDa
Purity95%

Storage & Handling

Storage-20°C
Form/AppearancePowder
Buffer/Preservatives25 mM MES, 150 mM NaCl, pH 5.5
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqQ9Y286

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide