Human Soluble Cluster of Differentiation 40 Ligand (sCD40L) ELISA Kit

SKU: orb866982

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Description

Sandwich Human sCD40L ELISA Kit

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sCD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sCD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sCD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sCD40L in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	6.6 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD154 ELISA Kit, TRAP ELISA Kit, HIGM1 ELISA Kit, IGM ELISA Kit, IMD3 ELISA Kit, TBAM ELISA Kit, T-BAM ELISA Kit, TNFSF5 ELISA Kit, Gp39 ELISA Kit, TNF Superfamily Member 5 ELISA Kit, Hyper-IgM Syndrome ELISA Kit, TNF-Related Activation Protein ELISA Kit, T-Cell B-Cell Activating Molecule ELISA Kit

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Human Soluble Cluster of Differentiation 40 Ligand (sCD40L) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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