Human Soluble Terminal Complement Complex C5b-9 (sC5b-9) ELISA Kit

SKU: orb2667219

Description

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research, Signal Transduction

Images & Validation

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Application Notes

standard: 2000 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sC5b-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sC5b-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sC5b-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sC5b-9 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 ng/mL
Sensitivity	12.9 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MAC; Membrane Attack Complex; C5b-9; Terminal Complement Complex C5b-9; C5b9

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