Human SPARC related modular calcium binding 1 (SMOC1) ELISA Kit

SKU: orb1146834

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPARC related modular calcium binding 1(SMOC1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SPARC related modular calcium binding 1(SMOC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPARC related modular calcium binding 1(SMOC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPARC related modular calcium binding 1(SMOC1) in the samples is then determined by comparing the OD of the samples to the standard curve.

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Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMOC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMOC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMOC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SMOC1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	22.5 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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SMOC1

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Human SPARC related modular calcium binding 1 (SMOC1) ELISA Kit

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Q9H4F8

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