Human Stathmin 1 (STMN1) ELISA Kit

SKU: orb779331

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stathmin 1(STMN1).

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STMN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STMN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STMN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STMN1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.056 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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LAP18 ELISA Kit, C1orf215 ELISA Kit, Lag ELISA Kit, OP18 ELISA Kit, PP17 ELISA Kit, PP19 ELISA Kit, PR22 ELISA Kit, SMN ELISA Kit, Metablastin ELISA Kit, Prosolin ELISA Kit, Oncoprotein 18 ELISA Kit, Leukemia-associated phosphoprotein p18 ELISA Kit

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UniProt

P16949

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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