Human T-Cell, Immune Regulator 1 (TCIRG1) ELISA Kit

SKU: orb780062

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell, Immune Regulator 1(TCIRG1).

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TCIRG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TCIRG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TCIRG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TCIRG1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	6 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Stv1 ELISA Kit, Vph1 ELISA Kit, ATP6N1C ELISA Kit, ATP6V0A3 ELISA Kit, Atp6i ELISA Kit, OC-116kDa ELISA Kit, OC116 ELISA Kit, OPTB1 ELISA Kit, TIRC7 ELISA Kit, a3 ELISA Kit, V-Type Proton ATPase 116 kDa Subunit A Isoform 3 ELISA Kit, Osteoclastic proton pump 116 kDa subunit ELISA Kit

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Human T-Cell, Immune Regulator 1 (TCIRG1) ELISA Kit

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UniProt

Q13488

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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