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Human TIM-3 Protein, Fc-Avi Tag (Biotin)

SKU: orb1742993

Description

Biotinylated Human TIM-3 Protein, Fc, is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Arg 200 (Accession # Q8TDQ0-1).

Research Area

Immunology & Inflammation

Images & Validation

Application Notes
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag . The protein has a calculated MW of 48.0 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
TargetTIM-3
ReactivityHuman
TagC-Fc & C-Avi
Expression RegionSer 22 - Arg 200
ConjugationBiotin
MW48.0 kDa
Purity90%

Storage & Handling

Storage-20°C
Form/AppearancePowder
Buffer/Preservatives50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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  • Recombinant Human TIM-3 Protein (Biotin) [orb2994140]

    Biotin

    SDS-PAGE: Greater than 85% as determined by reducing SDS-PAGE.

    Predicted: 48.8 KDa. Observed: 60-75 KDa, reducing conditions

    20 μg, 100 μg
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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqQ8TDQ0

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide