Human Titin (TTN) ELISA Kit

SKU: orb776002

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Titin(TTN).

Research Area

Musculoskeletal & Connective Tissue Research, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TTN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.118 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CMD1G ELISA Kit, CMH9 ELISA Kit, CMPD4 ELISA Kit, HMERF ELISA Kit, LGMD2J ELISA Kit, TMD ELISA Kit, Cardiomyopathy,Dilated 1G(Autosomal Dominant) ELISA Kit

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Quick Database Links

UniProt

Q8WZ42

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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