Human Transmembrane Protein 173 (TMEM173) ELISA Kit

SKU: orb781434

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 173(TMEM173).

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TMEM173. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMEM173. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TMEM173, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMEM173 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.065 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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NET23 ELISA Kit, ERIS ELISA Kit, MPYS ELISA Kit, MITA ELISA Kit, STING ELISA Kit, Endoplasmic Reticulum Interferon Stimulator ELISA Kit, Stimulator Of Interferon Genes Protein ELISA Kit, Mediator of IRF3 activation ELISA Kit

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Human Transmembrane Protein 173 (TMEM173) ELISA Kit

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UniProt

Q86WV6

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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