Human Tumor Protein p53 (TP53) ELISA Kit

SKU: orb775371

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein p53(TP53).

Research Area

Cancer Biology, Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TP53. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TP53. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TP53 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	78.13-5000 pg/mL
Sensitivity	27 pg/mL
Concentration	5000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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p53 ELISA Kit, LFS1 ELISA Kit, TRP53 ELISA Kit, Li-Fraumeni Syndrome ELISA Kit, Cellular tumor antigen p53 ELISA Kit, Antigen NY-CO-13 ELISA Kit, Phosphoprotein p53 ELISA Kit, Tumor suppressor p53 ELISA Kit

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Human Tumor Protein p53 (TP53) ELISA Kit

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UniProt

P04637

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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