Human Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit

SKU: orb775615

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3A(UBE3A).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UBE3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UBE3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UBE3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UBE3A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.122 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ANCR ELISA Kit, AS ELISA Kit, E6-AP ELISA Kit, EPVE6AP ELISA Kit, HPVE6A ELISA Kit, Papilloma Virus E6-Associated,Angelman Syndrome ELISA Kit, Oncogenic protein-associated protein E6-AP ELISA Kit, Renal carcinoma antigen NY-REN-54 ELISA Kit

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Human Ubiquitin Protein Ligase E3A (UBE3A) ELISA Kit

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UniProt

Q05086

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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