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IGF2R Antibody
SKU: orb1260633
Featured
Description
Research Area
Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 2327-2491 of human IGF2R (NP_000867.2). |
| Target | IGF2R |
| Molecular Weight | Observed: 310kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−IGF2R, CD222 antigen, CIMPR, CI Man-6-P receptor, CI-MPR, IGF-II receptor, MPR 300, MPR1, MPRI, M6P-R, M6P/IGF2R, CD222, M6P/IGF2 receptor
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Western blot analysis of extracts of HeLa cells, using IGF2R antibody (orb1260633) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human placenta using IGF2R antibody (orb1260633) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using IGF2R antibody (orb1260633) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using IGF2R antibody (orb1260633) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using IGF2R antibody (orb1260633) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using IGF2R antibody (orb1260633) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
IGF2R
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IGF2R Antibody (orb1260633)
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