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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli. |
| Target | IL1RN |
| Purity | This is an IgG preparation of whole rabbit serum purified by DEAE chromatography. In general, this antibody also detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra. The antibody does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II. This antibody is primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra) but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra). IcIL-1Ra is found in many cells as constitutively expressed protein, particularly epithelial cells and keratinocytes. This antibody does not distinguish between sIL-1Ra and icIL-1Ra in ELISA formats or by immunohistochemistry. When used in immunoblot analysis, it will recognize 17,000 MW IL-1Ra in supernatants released from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS. However, in lysates of PBMC, icIL-1Ra is also found after 48-72 hours of culture. In monocytic cells, the initial IL-1Ra transcript is for sIL-1Ra which can be found almost exclusively intracellularly. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−IL1RA antibody, IL1RN antibody, IL1RT1 antibody, Interleukin 1 receptor alpha type 1 antibody, Interleukin 1 receptor antagonist protein (IRAP) antibody, rabbit anti-IL-1 Receptor Antagonist Antibody, rabbit anti-1 receptor antagonist protein antibody, rabbit anti-IRAP antibody, IL1F3, IL1RA, ICIL-1RA, IL1 inhibitor, INN: Anakinra, IL-1ra, IL-1RN, Interleukin-1 receptor antagonist protein
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Western Blot of Rabbit Anti-Human IL-1 Receptor Antagonist Antibody. Lane 1: Recombinant Human IL-1 RA Protein [0.05 µg] Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Recombinant Human IL-1 RA Protein [0.05 µg] Non-Reduced. Primary Antibody: Anti-Hu IL-1RA Antibody at 1:800 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Antibody (p/n orb347673) at 1:40000 for 30 mins at RT. Blocking Buffer: BlockOut Universal Buffer (p/n orb348644) for 1hr at RT. Excepted Molecular Weight: ~18kDa. Exposure: 7.193 secs.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL1RN Antibody (orb345123)
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