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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IP, WB |
|---|---|
| Dilution Range | ELISA: 1:1,000 - 1:2,000, IP: 1:400 - 1:800, WB: 1:500 - 1:2,000 |
| Reactivity | Human, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-1R type II. |
| Target | IL1R2 |
| Purity | This antiserum is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RII. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type I. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type II (also called soluble IL-1R type II) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 85 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−IL1RB antibody, Interleukin 1 receptor type II precursor antibody, MGC47725 antibody, p68 antibody, Type II interleukin 1 receptor beta antibody, Antigen CDw121b antibody, rabbit anti-interleukin-1 Receptor Type II Antibody
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Western blot using Biorbyt's anti-IL-1RII antibody. This blot shows detection of an IL-1RII-GST fusion protein (~500 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown). Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000. Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown for size comparison (lane M, red, 700 nm channel).
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
IL1R2 Antibody (orb750674)
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