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IL-10 Antibody

SKU: orb1538986

Description

IL-10 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution rangeELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
ReactivityHuman
Application Notes
Resuspension: Reconstitute in sterile water to 0.1-1 mg/ml. . Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-10 (1 ng/ml), a concentration of 1.2-1.8 ug/ml of this antibody is required. ELISA: To detect hIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-10 as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenE.coli derived recombinant Human IL-10.
TargetIL-10
PurificationImmunoaffinity purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLyophilized from PBS, pH 7.2
DisclaimerFor research use only

Alternative Names

IL10, CSIF, GVHDS, IL-10, IL10A, Interleukin-10, Interleukin 10, TGIF

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IL-10 Antibody (orb1538986)

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Available Sizes

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50 μg
¥ 8,320.00
DispatchUsually dispatched within 2-3 weeks
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