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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-17E (human IL-17E). |
| Target | IL25 |
| Purification | Anti-hIL-17E specific antibody was purified by affinity chromatography employing immobilized hIL-17E matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−IL17E, IL17E, UNQ3120/PRO10272, Interleukin-25, Interleukin-17E, IL-25
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IL25 Antibody [orb1410141]
IHC
Human
Mouse
Monoclonal
Unconjugated
20 μg, 100 μg, 100 μg (without BSA and Azide)
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To detect Human IL-17E by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human IL-17E (orb1272431) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17E.
To detect Human IL-17E by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17E is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL25 Antibody (orb1272432)
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