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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000-1:50,000, FC: 1µg/mL, WB: 0.5µg/mL |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 11K8B1 |
| Immunogen | Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development. |
| Target | IL25 |
| Purity | Anti-IL-17E was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17E protein. A BLAST analysis was used to suggest cross-reactivity with IL-17E from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17E from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse Anti-IL-17E Antibody, mouse anti-IL-25 antibody, Interleukin 25, IL-25, Interleukin 17E, IL-17E
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IL25 Antibody [orb1410141]
IHC
Human
Mouse
Monoclonal
Unconjugated
20 μg, 100 μg, 100 μg (without BSA and Azide)
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Flow Cytometry of Mouse anti-IL-17E antibody. Cells: PC3 cells. Primary antibody: 1 ug of IL-17E (red), control (green). Secondary antibody: anti-mouse IgG PE conjugated secondary antibody.
Western Blot of Mouse anti-IL-17E antibody in full length recombinant protein using IL-17E antibody at 0.5 ug/ml.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_073626.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL25 Antibody (orb345112)
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