IL25 Antibody (Biotin)

SKU: orb345120

This product can be custom made

Description

IL-17E antibody (Biotin)

Research Area

Cell Biology

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, FC, WB
Dilution Range	ELISA: 1:10,000 - 1:50,000, FC: 1µg/mL, WB: 0.5µg/mL
Reactivity	Human
Application Notes	This purified antibody has been tested for use in Flow Cytometry and western blotting. Specific conditions for reactivity should be optimized by the end user.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	11K8B1
Immunogen	Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.
Target	IL25
Purity	This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17E protein. A BLAST analysis was used to suggest cross-reactivity with IL-17E from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17E from other sources has not been determined.
Conjugation	Biotin

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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mouse Anti-IL-17E Biotin Conjugated Antibody, mouse anti-IL-25 biotin conjugated antibody, Interleukin 25, IL-25, Interleukin 17E, IL-17E

Quality Guarantee

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Anti-IL-17E Antibody - Flow Cytometry Cells: PC3 cells Primary antibody: 1 ug of IL-17E (red), control (green) Secondary antibody: anti-mouse IgG PE conjugated secondary antibody.

Anti-IL-17E Biotin Conjugated Antibody - Western Blot. Western Blot of Mouse anti-IL-17E antibody. Load: full length recombinant protein. Primary Antibody: anti-IL-17E antibody (orb345112) at 0.5 ug/ml.

Quick Database Links

Gene Symbol

IL25

UniProt

Q9H293

RefSeq (Protein):NP_073626.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_073626.1

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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