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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | DOT, ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000, WB: 1:1,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein. |
| Target | Il17f |
| Purity | IL-17F antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse IL17-F in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store anti-IL-17F antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: None. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-IL-17F antibody, rabbit anti-interleukin-17F antibody, Interleukin17F, Interleukin-17F, ML1, ML-1, IL17F, IL24
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Western blot using Biorbyt's anti-Mouse IL-17F antibody shows detection of a band ~18 kDa in size corresponding to recombinant mouse IL-17F. Recombinant mouse IL-17F was loaded on to an SDS-PAGE gel at 0.25 µg and after separation was transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (p/n orb348637) for 30 min at RT followed by reaction with FemtoMax™ Chemiluminescent substrate.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_665855.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Il17f Antibody (orb345222)
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