IL17F Antibody (HRP)

SKU: orb345158

This product can be custom made

Description

IL-17F antibody (Peroxidase)

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:10,000 - 1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
Reactivity	Human
Application Notes	This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 15.0 kDa in size corresponding to human IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL17-F protein.
Target	IL17F
Purity	This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL17-F in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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rabbit anti-IL-17F peroxidase conjugated antibody, rabbit anti-Interleukin-17F HRP conjugated antibody, Cytokine ML-1, Interleukin-24

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western Blot of Peroxidase conjugated Rabbit anti-IL-17F Antibody. Lane 1: Human IL-17F. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase IL-17F secondary antibody at 1:1000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 16kDa for Human IL-17F. Other band(s): none.

Quick Database Links

Gene Symbol

IL17F

UniProt

Q96PD4

RefSeq:AAH662531.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	AAH662531.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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