IL17F Antibody (Biotin)

SKU: orb345116

Description

Human IL-17F antibody (Biotin)

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:10,000-1:50,000, IHC: 5µg/mL, WB: 0.5 mg/mL
Reactivity	Human
Application Notes	This purified antibody has been tested for use in IHC and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	4H1629.1
Immunogen	Anti-IL-17F (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17F produced in E.coli followed by hybridoma development.
Target	IL17F
Purity	This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human IL-17F protein. A BLAST analysis was used to suggest cross-reactivity with IL-17F from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-17F from other sources has not been determined.
Conjugation	Biotin

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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mouse anti-IL-17F biotin conjugated antibody, mouse anti-Interleukin-17F biotin conjugated antibody, Cytokine ML-1, Interleukin-24

Quality Guarantee

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Immunohistochemistry of Mouse Anti-IL-17F antibody. Tissue: human colon tissue. Fixation: formalin-fixed, paraffin-embedded. Primary antibody: isotype control (top left), Mouse Anti-IL-17F antibody (right and bottom left) at 5 ug/ml.

Western Blot of Mouse Anti-IL-17F antibody. Lane 1: human full length recombinant IL-17F protein. Lane 2: mouse full length recombinant IL-17F protein. Lane 3: rat full length recombinant IL-17F protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17F antibody (orb345104) at 0.5 ug/ml for overnight at 4°C.

Quick Database Links

Gene Symbol

IL17F

UniProt

Q96PD4

RefSeq (Protein):NP_443104.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_443104.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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