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IL1RN Antibody (Biotin)

SKU: orb1272756

Description

IL1RN Antibody (Biotin)

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
ELISA:Sandwich:To detect Human IL-1RA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-1RA as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA. Western Blot:To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenProduced from sera of rabbits immunized with highly pure recombinant Human IL-1RA. Human IL-1RA specific antibody was purified by affinity chromatography and then biotinylated.
TargetIL1RN
ConjugationBiotin

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

DIRA, IRAP, IL1F3, IL1RA, MVCD4, IL-1RN, IL-1ra, IL-1ra3, ICIL-1RA, Interleukin-1 receptor antagonist protein

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IL1RN Antibody (Biotin)

To detect Human IL-1RA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-1RA (orb1272616) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-1RA.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP18510

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IL1RN Antibody (Biotin) (orb1272756)

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Available Sizes

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0.05 mg
¥ 10,140.00
Free Secondary Antibody (20 ul)0/0

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