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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Produced in mice immunized with highly pure Recombinant Human IL-3. Anti-Human IL-3-specific antibody was purified from tissue culture supernatant by Protein G affinity chromatography. |
| Target | IL3 |
| Purification | IL-3-specific antibody was purified from tissue culture supernatant by Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-3, MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor
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In a sandwich ELISA (assuming 100 μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 8 pg/ml of Recombinant Human IL-3 when used with Biotinylated Antigen Affinity Purified Anti-Human IL-3 as the detection antibody at a concentration of approximately 0.50- 1.0 μg/ml.
To detect Human IL-3 by Western Blot analysis, this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-3 is 2.0-4.0 ng/lane, under reducing and non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL3 Antibody (orb1671479)
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