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Description
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−Item 1 of 1
| Tested Applications | ELISA, IHC, IP, WB |
|---|---|
| Dilution Range | ELISA: 1:1,000 - 1:5,000, IHC: 1:400 - 1:800, IP: 1:400 - 1:800, WB: 1:500 - 1:2,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli. |
| Target | IL8 |
| Purity | Anti-IL 8 antiserum has been heated to 56°C for 30 minutes. The antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA. Although likely to be functional, this antiserum has not been evaluated in other assays, including neutralization and immunohistochemistry. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store anti-IL8 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | None |
| Concentration | 75 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−Anti-CXCL 8, CXCL8 antibody, 310C antibody, AMCF 1 antibody, AMCF1 antibody, b ENAP antibody, Beta thromboglobulin like protein antibody, CXC chemokine ligand 8 antibody, interleukin8 antibody, K60 antibody, LECT antibody, LUCT antibody, rabbit anti-Interleukin 8 Antibody, rabbit anti-IL-8
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Western Blot of Rabbit anti-Human IL-8 Antibody. Lane 1: Recombinant Human IL-8. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human IL-8 Antibody 1:1000 overnight at 4°C. Secondary antibody: HRP Goat-a-rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 11 kDa, 11 kDa for Human IL-8.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
IL8 Antibody (orb750683)
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