IL13 Antibody

SKU: orb1538972

This product can be custom made

Description

IL13 Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P, WB
Dilution Range	ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
Reactivity	Human
Application Notes	Resuspension: Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. . Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-13 (2 ng/ml), a concentration of 0.12-0.15 ug/ml of this antibody is required. ELISA: To detect hIL-13 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-13 as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-13. Western Blot: To detect hIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-13 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. Read more...

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	E.coli derived recombinant Human IL-13.
Target	IL13
Purification	Immunoaffinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Lyophilized from PBS, pH 7.2
Concentration	0.1 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IL13, ALRH, BHR1, Interleukin-13, NC30, p600, IL-13, Interleukin 13

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Gene Symbol

IL13

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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IL13 Antibody (orb1538972)

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Reactivity:	Equine
Dynamic Range:	7.82-500 pg/mL
Sensitivity:	3.2 pg/mL

Reactivity:	Human
Dynamic Range:	15.63-1000 pg/mL
Sensitivity:	6.4 pg/mL

Applications:	ELISA, FC, IHC, WB
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IHC-P
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Reactivity:	Mouse
Dynamic Range:	0.32-20 ng/mL
Sensitivity:	0.126 ng/mL

IL13 Antibody

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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Quality Guarantee

Quick Database Links

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Protocol Information

IL13 Antibody (orb1538972)

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