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IL2 Antibody
SKU: orb1259059
Featured
Description
Research Area
Cell Biology, Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 6
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human IL2 |
| Target | IL2 |
| Molecular Weight | Observed: 18kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine
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Western blot analysis of extracts of various cell lines, using IL2 antibody (orb1259059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of Raw264.7 cells, using IL2 antibody (orb1259059) at 1:1000 dilution. Raw264.7 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using IL2 antibody (orb1259059) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
IL2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IL2 Antibody (orb1259059)
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