IL2RA/CD25 Antibody

SKU: orb1538961

Description

IL2RA/CD25 Antibody

Images & Validation

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Tested Applications	ELISA, IHC, IHC-Fr, IHC-P, WB
Dilution range	ELISA (4 - 8 µg/ml), IHC, IHC-Fr (5 µg/ml), IHC-P (10 µg/ml), WB (0.2 - 0.4 µg/ml)
Reactivity	Human
Application Notes	Resuspension: Centrifuge vial prior to opening. Reconstitiute in sterile water to 1.0 mg/ml.. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Ralpha when used in conjunction with compatible secondary reagents. Western Blot: To detect Human sIL-2Ralpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Ralpha is 2-4 ng/lane, under non-reducing conditions. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a,k
Immunogen	Insect cell derived recombinant Human sIL-2Ralpha.
Target	IL2RA / CD25
Purification	Protein G purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Lyophilized from Sterile Solution PBS
Disclaimer	For research use only

Alternative Names

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IL2RA, CD25 antigen, CD25, IDDM10, IL-2 receptor subunit alpha, IL2R, Interleukin 2 receptor, alpha, IL-2-RA, IL2-RA, TCGFR, IL-2R subunit alpha, p55, TAC antigen

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Gene Symbol

IL2RA / CD25

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Datasheet

Product Information

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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