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IM-12

SKU: orb1305870

Description

IM-12 is a potent and selective small molecule inhibitor of GSK-3β with an IC₅₀ of 53 nM. It acts as a canonical Wnt/β-catenin pathway activator and is a valuable chemical tool for studying Wnt signalling in stem cell biology, oncology, and neurological research, both in vitro and in vivo.

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

Key Properties

CAS Number1129669-05-1
MW377.41
Purity99.85%
FormulaC22H20FN3O2
SMILESCN1C(=O)C(NCCc2ccc(F)cc2)=C(C1=O)c1c(C)[nH]c2ccccc12
TargetWnt/beta-catenin,GSK-3
SolubilityH2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:10 mg/mL (26.5 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.3 mM);DMSO:65 mg/mL (172.23 mM)

Bioactivity

Target IC50
GSK-3β:53 nM
In Vivo
In human neural progenitor cells (hNPCs) with an IC50 of 3.8 μM, IM-12 inhibits GSK-3β activity and increases the concentration of β-catenin. IM-12 promotes cell proliferation in human neural progenitor cells by activating the Wnt signaling pathway.
Cell Research
To measure viable cells, 50-100 μL of cell suspension is analyzed using CASY technology with the appropriate program. ReNcell VM cells are seeded at a defined cell number and proliferated for 24 h. Then the medium is changed to proliferation medium with added substances at indicated concentrations. The cell number was determined every 24 h. Cells were exposed to the added drugs during the whole experiment, whereas the media is changed every 24 h.(Only for Reference)

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Inhibitor, Glycogen synthase kinase 3, Glycogen synthase kinase-3, GSK-3β, GSK3, GSK-3, inhibit, IM-12, IM 12, IM12

Similar Products

  • IM-12 [orb1226948]

    >98% (HPLC)

    1129669-05-1

    377.4

    C22H20FN3O2

    1 g, 50 mg, 10 mg, 100 mg, 500 mg, 25 mg, 5 mg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Key Properties

No computed properties available.

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IM-12 (orb1305870)

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Step 1: Enter information below

(Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

2 mg
¥ 1,040.00
5 mg
¥ 1,170.00
1 ml x 10 mM (in DMSO)
¥ 1,300.00
10 mg
¥ 1,560.00
25 mg
¥ 2,600.00
50 mg
¥ 3,770.00
100 mg
¥ 5,460.00
500 mg
¥ 14,690.00
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