INHA Antibody (N-term)

SKU: orb1929850

Description

Research Area

Cancer Biology, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-P, WB
Dilution range	WB - 1:2000
Reactivity	Human, Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	39670 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Quality Guarantee

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INHA Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of INHA Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

INHA Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INHA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-INHA Antibody (N-term) at 1:2000 dilution. Lane 1: SK-BR-3 whole cell lysate. Lane 2: Mouse ovary whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P05111

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunofluorescence