Interferon alpha 2b Rabbit Polyclonal Antibody

SKU: orb13483

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Description

Research Area

Immunology

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human IFN alpha 2b
Target	IFNA2
Molecular Weight	19 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Interferon alpha-2b; alpha 2a interferon; alpha-2a interferon; IFN alpha 2b; IFNA 2; IFNA; INFA 2; INFA2; Interferon alpha 2; Interferon alpha 2 precursor; Interferon alpha 8 precursor; Interferon alpha A; Interferon alpha B; Interferon alpha B2; Interferon alpha-2 precursor; LeIF A; LeIF B; LeIFA; MGC125764; Interferon alpha B; Interferon alpha B2; interferon, alpha 8; LeIF B; MGC125765; IFN-alpha-2; IFNA2_HUMAN; MGC125765.

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Interferon alpha 2b Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Interferon alpha 2b) Polyclonal Antibody, Unconjugated (orb13483) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Recombinant human Interferon alpha 2 protein, N-His, Primary: Anti-Interferon alpha 2b (orb13483) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 19 kDa, Observed band size: 16 kDa.

Tissue/Cell: mouse liver tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IFN-Alpha 2b/Interferon alpha 2/INFA2 Polyclonal Antibody, Unconjugated (orb13483) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

IFNA2

UniProt

P01563

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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