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IRF-3 (phospho Ser385) rabbit pAb Antibody

SKU: orb768798
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Description

Rabbit polyclonal antibody to IRF-3 (phospho-Ser385)

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
ReactivityHuman, Mouse, Rat

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser385. AA range:351-400
Molecular Weight57
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-IRF3 antibody, anti-Interferon regulatory factor 3 antibody, anti-IRF-3 antibody
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

IRF-3 (phospho Ser385) rabbit pAb Antibody

Immunofluorescence analysis of Hela cell. 1, IRF-3 (phospho Ser385) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). COX IV Monoclonal Antibody (6C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

IRF-3 (phospho Ser385) rabbit pAb Antibody

Western Blot analysis of 293 cells using Phospho-IRF-3 (S385) Polyclonal Antibody.

IRF-3 (phospho Ser385) rabbit pAb Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRF-3 (Phospho-Ser385) Antibody.

IRF-3 (phospho Ser385) rabbit pAb Antibody

Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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IF
Immunofluorescence
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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IRF-3 (phospho Ser385) rabbit pAb Antibody (orb768798)

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100 μl
¥ 7,410.00
DispatchUsually dispatched within 5-10 working days
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