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IRF-3 (phospho Ser385) rabbit pAb Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser385. AA range:351-400 |
| Molecular Weight | 57 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunofluorescence analysis of Hela cell. 1, IRF-3 (phospho Ser385) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). COX IV Monoclonal Antibody (6C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of 293 cells using Phospho-IRF-3 (S385) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRF-3 (Phospho-Ser385) Antibody.

Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) Antibody. The picture on the right is blocked with the phospho peptide.
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Protocol Information
IRF-3 (phospho Ser385) rabbit pAb Antibody (orb768798)
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