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ITM2B Antibody

SKU: orb1249554

Description

ITM2B Antibody

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 30-35kDa band observed in HepG2 cell lysates (calculated MW of 30.3kDa according to NP_068839.1). Recommended concentration: 0.1-0.3µg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 2.5µg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-HDKETYKLQRRETIK
TargetITM2B
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ITM2B, integral membrane protein 2B, ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD, BRICHOS domain containing 2B, OTTHUMP00000018394

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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