KCNN4/KCa3.1 Antibody

SKU: orb1538492

Description

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC, IHC-P, WB
Dilution range	IF (10 µg/ml), IHC, IHC-P (3.75 - 7.5 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml)
Reactivity	Bovine, Canine, Human, Rabbit

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-ERQAVNATGHLSD, from the internal region of the protein sequence according to NP_002241.1.
Target	KCNN4 / KCa3.1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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KCNN4, HIKCa1, HKCa4, HIK1, IK1, KCA4, HSK4, IKCA1, KCa3.1, SK4, SKCa 4, Putative Gardos channel, SKCa4

Quality Guarantee

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Anti-KCNN4 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

Goat Anti-KCNN4 Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Goat Anti-KCNN4 Antibody (0.1 µg/ml) staining of Human Cerebellum (A) + Peptide (B), NIH3T3 (C) and negative Control Adrenal Gland (D) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescencence.

Goat Anti-KCNN4 Antibody Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton.

Quick Database Links

Gene Symbol

KCNN4 / KCa3.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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