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Kir2.1 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | AM, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB (1:1000), IHC-P (1:1000), ICC/IF (1:100) |
| Reactivity | Human, Monkey, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG1 |
| Clone No. | S112 |
| Immunogen | Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1 |
| Target | Kir2.1 |
| Molecular Weight | 45kDa |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Kir2.1 Rabbit Polyclonal Antibody [orb184788]
FC, WB
Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit, Sheep
Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl, 50 μl, 100 μlKIR2.1 rabbit pAb Antibody [orb765550]
ELISA, IF, IHC, WB
Human, Rat
Polyclonal
Unconjugated
100 μl, 50 μlKir2.1 Antibody (FITC) [orb148332]
AM, ICC, IF, IHC, WB
Human, Monkey, Mouse, Rat
Mouse
Recombinant
FITC
100 μgKir2.1 Antibody (HRP) [orb148333]
AM, ICC, IF, IHC, WB
Human, Monkey, Mouse, Rat
Mouse
Recombinant
HRP
100 μgKir2.1 Antibody (RPE) [orb148336]
AM, ICC, IF, IHC, WB
Human, Monkey, Mouse, Rat
Mouse
Recombinant
RPE
100 μg

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir2.1 Monoclonal Antibody, Clone S112. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir2.1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.

Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
UniProt Details
−NCBI Gene Details
−NCBI Reference Sequences
−| Protein | NP_032451 |
|---|
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Protocol Information
Kir2.1 Antibody (orb67400)
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