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KO/KD
Validated
Validated
Description
Research Area
Neuroscience, Signal Transduction
Images & Validation
−
| Tested Applications | FC, IHC-P, KO/KD Validated, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 FC: 1/50-1/100 |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | B5D6 |
| Immunogen | Full length human recombinant protein of human AKT1 produced in 293T cell. |
| Target | AKT1 |
| Molecular Weight | Calculated MW: 56 kDa; Observed MW: 60 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in PBS containing 50% glycerol, 1% rAlbumin and 0.02% sodium azide, pH 7.3. |
| Buffer/Preservatives | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha
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Quick Database Links
Gene Symbol
AKT1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
Available Sizes
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Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days